Anti-Aquaporin 2 (Ser269) Antibody

28 Citations

Our Anti-Aquaporin 2 (Ser269) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser269) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.

Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).
Western analysis of rat kidney inner medullary (IM) lysate from ddAVP and ddAVP + ADM-treated IM probed for total (bottom) and pSer269 (cat. p112-269, 1:1000)(top) AQP2. Brackets indicate the glycosylated AQP2 protein between 35 and 45 kDa, and the arrow indicates the un glycosylated AQP2 protein at 29 kDa. The matched pair comparisons were achieved by comparing Lane 1 with Lane 5; Lane 2 with Lane 6; Lane 3 with Lane 7; and Lane 4 with Lane 8. Image from publication CC-BY-4.0. PMID: 37047509
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).
Western analysis of rat kidney inner medullary (IM) lysate from ddAVP and ddAVP + ADM-treated IM probed for total (bottom) and pSer269 (cat. p112-269, 1:1000)(top) AQP2. Brackets indicate the glycosylated AQP2 protein between 35 and 45 kDa, and the arrow indicates the un glycosylated AQP2 protein at 29 kDa. The matched pair comparisons were achieved by comparing Lane 1 with Lane 5; Lane 2 with Lane 6; Lane 3 with Lane 7; and Lane 4 with Lane 8. Image from publication CC-BY-4.0. PMID: 37047509
Human, Mouse, Rat
ICC, IHC, IP, WB
Rabbit

Go to Full Product Details

from PhosphoSolutions Datasheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p112-269

Volume: 100 µL
Price:
$430

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
33436646 1:200 Flahaunt, A., et al. 2021. A metabolically stable apelin-17 analog decreases AVP-induced antidiuresis and improves hyponatremia. Nature Communications, 12(1), pp.1-14.
Immunocytochemistry: Rat
PMID Dilution Publication
28668390 1:500 Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044.
Immunohistochemistry: Mouse
PMID Dilution Publication
18606813 1:2000 Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
Immunohistochemistry: Rat
PMID Dilution Publication
26147297 not listed Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
23559583 1:600 Bogum, J., et al. 2013. Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.
Immunoprecipitation: Mouse
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
Immunoprecipitation: Rat
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
Western Blot: Human
PMID Dilution Publication
32529500 not listed Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
32486031 not listed Ranieri, M., et al. 2020. The Vasopressin Receptor 2 Mutant R137L Linked to the Nephrogenic Syndrome of Inappropriate Antidiuresis (NSIAD) Signals through an Alternative Pathway that Increases AQP2 Membrane Targeting Independently of S256 Phosphorylation. Cells, 9(6), p.1354.
Western Blot: Mouse
PMID Dilution Publication
369959261:1000Zuchowski, Y., et al. 2023. The Insulin Regulated Aminopeptidase (IRAP) is Required for Water Excretion in Response to Acute Hypotonic Stress. American Journal of Physiology. Renal Physiology, F521-F531.
363268351:1000Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .
32529500 not listed Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
29046300 1:3000 Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
27889609 1:500-1:1000 Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27707708 1:1000 Gao, Y., et al. 2016. Collecting duct-specific knockout of nitric oxide synthase 3 impairs water excretion in a sex-dependent manner. American Journal of Physiology-Renal Physiology, pp.ajprenal-00494.
18606813 1:2000 Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
Western Blot: Rat
PMID Dilution Publication
370475091:1000Wang, Y., et al. 2023. Phosphatases Decrease Water and Urea Permeability in Rat Inner Medullary Collecting Ducts. International Journal of Molecular Sciences, .
31994353 1:1000 Masuda, T., et al. 2020. Osmotic diuresis by SGLT2 inhibition stimulates vasopressin‐induced water reabsorption to maintain body fluid volume. Physiological Reports, 8(2), p.e14360.
29046300 1:3000 Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
28668390 1:500 Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044.
27889609 1:500-1:1000 Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27534994 not listed Al-Butaineh, M.M, et al. 2016. Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292 not listed Speed, J.S, et al. 2016. In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26574044 1:5000 Mamenko, M., et al. 2016. Defective store-operated calcium entry causes partial nephrogenic diabetes insipidus. Journal of the American Society of Nephrology, 27(7), pp.2035-2048.
26147297 not listed Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
25651562 1:1000 Choi,H. J., et al. 2015. Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308(7):F737-48.
24259510 1:1000 Hoffert, J.D., et al. 2014. Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421.
23559583 1:600 Bogum, J., et al. 2013. Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.

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Product Specific Protocols

  • Aquaporin ser269 IHF Protocol: Download

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