Secondary Antibody Issues are Often Overlooked in Immunochemistry

The Choice of Secondary Antibody is one of the Most Overlooked Issues in Immunochemistry. After the tissue of interest has been incubated with the primary antibody, the next critical step involves incubating the tissue in a labeled secondary antibody to detect the presence of the primary antibody. The choice of the secondary antibody has to […]

Why Don’t More Researchers Validate Antibodies with WB Before Doing IHC?

  As we have discussed previously in this blog, there is widespread concern about antibody validation. Of course some forms of validation using multiple antibodies or knockouts are not available for the majority of targets of interest to researchers.  But western blots are a excellent form of validation that can be readily performed by almost […]

Antibodies That DO NOT WORK in Western Blots

    What does it mean when an antibody company says an antibody “does not work” in western blots? I started wondering about this question the other day after a colleague told me about a company that “validates” all of their antibodies by immunostaining in no less than 5 different tissues!  We examined the company’s […]