Why Don’t More Researchers Validate Antibodies with WB Before Doing IHC?

  As we have discussed previously in this blog, there is widespread concern about antibody validation. Of course some forms of validation using multiple antibodies or knockouts are not available for the majority of targets of interest to researchers.  But western blots are a excellent form of validation that can be readily performed by almost […]

Why Recombinant Proteins Make Poor Antibody Validation Tools

Antibody validation is a topic that has garnered a great deal of attention lately in discussions of the problem with the lack of reproducibility in science.  One type of antibody validation that should be avoided uses purified recombinant proteins.  In a typical study of this type a purified recombinant protein is run on a western […]