Our GluR1 Ser845 antibody is works great in WB and ICC!
Western blot of showing sepcific labeling of our GluR1 Ser845 and DARPP-32 Thr34 antibodies in rat striatal slice treatment with NMDA (25 μM, 5min), dopamine (DA, 10 μM, 15 min), or both on Cdk5-dependent phosphorylation. Read more about this image in the publication. Image from publication CC-BY-4.0.
Phosphorylation of GluR1 Ser845 is mediated by cAMP-dependent protein kinase A (PKA) or cGMP-dependent protein kinase II (cGKII, aka PRKG2).
AMPA receptors (AMPARs) mediate the majority of the fast excitatory synaptic transmission in the mammalian central nervous system (Diering and Huganir, 2018). AMPARs are comprised of four distinct glutamate receptor subunits designated (GluR1-4, also known as GluA1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994).
Phosphorylation of GluR1 Ser845 promotes GluR1 surface expression, increases channel open-probability, and stabilization of GluR1 on the cell-surface by enhancing GluR1 recycling and limiting endocytosis (Diering and Huganir, 2018). Additionally, GluR1 Ser845 phosphorylation plays a role in several forms of synaptic plasticity, including LTP and synaptic upscaling. In contrast, calcineurin-mediated dephosphorylation of GluR1 S845 is involved in receptor internalization during LTD and synaptic downscaling (Sathler et al., 2021).
Western blot of showing specific labeling of GluR1 Ser845 on a phosphatase treated rat hippocampus blot.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Several researchers have published with our GluR1 Ser845 antibody in the last year and a half!
|GluR1 Ser845 Publications, 2021-2022|
Thomas, R., et al. 2022. Integrated regulation of PKA by fast and slow neurotransmission in the nucleus accumbens controls plasticity and stress responses. Journal of Biological Chemistry, 298(8).
|35099830||Mouse||ICC||1:100||Sun, J., et al. 2022. LAMTOR1 inhibition of TRPML1-dependent lysosomal calcium release regulates dendritic lysosome trafficking and hippocampal neuronal function. The EMBO Journal, 41(5), p. e108119.|
|33853773||Mouse||WB||1:1000||Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300.|
|34592633||Rat||WB||1:1000||Del Angel, Y.C., et al. 2021. Down-regulation of AMPA receptors and long-term potentiation during early epileptogenesis. Epilepsy and Behavior, 124, p. 108320.|
- Diering GH, Huganir RL. (2018) The AMPA Receptor Code of Synaptic Plasticity. Neuron; 100(2):314-329.
- Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108.
- Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560.
- Sathler MF, Khatri L, Roberts JP, Schmidt IG, Zaytseva A, Kubrusly RC, Ziff EB, Kim S. (2021) Phosphorylation of the AMPA receptor subunit GluA1 regulates clathrin-mediated receptor internalization. J Cell Sci; 134 (17): jcs257972