PhosphoSolutions Joins Forces with Antibodies Incorporated and Aves Labs!

PhosphoSolutions is proud to announce its new partnership with Davis, CA-based Antibodies Incorporated and Aves Labs, two providers of highly-validated antibodies to the research community. About Antibodies Incorporated Founded in 1962, Antibodies Incorporated boasts a catalog of nearly 500 mouse monoclonal antibodies against critical neuroscience targets, many of which have been knockout validated in mouse […]

Secondary Antibody Issues are Often Overlooked in Immunochemistry

The Choice of Secondary Antibody is one of the Most Overlooked Issues in Immunochemistry. After the tissue of interest has been incubated with the primary antibody, the next critical step involves incubating the tissue in a labeled secondary antibody to detect the presence of the primary antibody. The choice of the secondary antibody has to […]

Why Don’t More Researchers Validate Antibodies with WB Before Doing IHC?

  As we have discussed previously in this blog, there is widespread concern about antibody validation. Of course some forms of validation using multiple antibodies or knockouts are not available for the majority of targets of interest to researchers.  But western blots are a excellent form of validation that can be readily performed by almost […]

Why Recombinant Proteins Make Poor Antibody Validation Tools

Antibody validation is a topic that has garnered a great deal of attention lately in discussions of the problem with the lack of reproducibility in science.  One type of antibody validation that should be avoided uses purified recombinant proteins.  In a typical study of this type a purified recombinant protein is run on a western […]

Antibodies That DO NOT WORK in Western Blots

    What does it mean when an antibody company says an antibody “does not work” in western blots? I started wondering about this question the other day after a colleague told me about a company that “validates” all of their antibodies by immunostaining in no less than 5 different tissues!  We examined the company’s […]

Are Monoclonal Antibodies Really More Specific?

It is common to see references to the “specificity” or “greater specificity” of monoclonal antibodies compared to polyclonal antibodies. Is that claim justified? No one would dispute the fact that a monoclonal is directed toward a single site or epitope of the target protein, while a polyclonal may contain antibodies directed toward multiple epitopes on […]

Antibody Specificity: The Use of a Blocking Control has only Limited Value

Antibody specificity:  The use of a blocking control has only limited value Antibody specificity is one of the key issues in determining whether you have an antibody that works.  How does one determine that the antibody specifically recognizes only the target of interest?  There are a number of control procedures one can use to be […]

Antibodies that work begins

  Antibodies that work are extremely valuable research tools.   However, the search for such antibodies is made quite difficult by two different problems.  The first problem, “the good antibody problem” is that production of a good antibody is a difficult, time consuming process; and many researchers and antibody companies do not possess either the patience […]