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Why Don’t More Researchers Validate Antibodies with WB Before Doing IHC?

  As we have discussed previously in this blog, there is widespread concern about antibody validation. Of course some forms of validation using multiple antibodies or knockouts are not available for the majority of targets of interest to researchers.  But western blots are a excellent form of validation that can be readily performed by almost […]

Why Recombinant Proteins Make Poor Antibody Validation Tools

Antibody validation is a topic that has garnered a great deal of attention lately in discussions of the problem with the lack of reproducibility in science.  One type of antibody validation that should be avoided uses purified recombinant proteins.  In a typical study of this type a purified recombinant protein is run on a western […]

Are Monoclonal Antibodies Really More Specific?

It is common to see references to the “specificity” or “greater specificity” of monoclonal antibodies compared to polyclonal antibodies.  Is that claim justified?  No one would dispute the fact that a monoclonal is directed toward a single site or epitope of the target protein, while a polyclonal may contain antibodies directed toward multiple epitopes on […]

Antibody Specificity: The Use of a Blocking Control has only Limited Value

Antibody specificity:  The use of a blocking control has only limited value Antibody specificity is one of the key issues in determining whether you have an antibody that works.  How does one determine that the antibody specifically recognizes only the target of interest?  There are a number of control procedures one can use to be […]

Antibodies that work begins

  Antibodies that work are extremely valuable research tools.   However, the search for such antibodies is made quite difficult by two different problems.  The first problem, “the good antibody problem” is that production of a good antibody is a difficult, time consuming process; and many researchers and antibody companies do not possess either the patience […]